October 12-23
From protein to gene, from gene to protein
Paula Veríssimo
Sandra Ribeiro
Sukalyan Chaterjee
Rui Cruz
Raquel Vinhas
Lab. Biotecnologia Molecular do CNC
Dep. Bioquímica, Universidade de Coimbra
IBMC
Lectures:
- Lect.1 Cloning strategies
- Lect.2 Expression of cloned genes in prokaryotes
- Lect. 3, 4: Expression of cloned genes in eukaryotes
- Lect. 5: Recombinant protein production
- Lect. 6: Protein purification
- Lect. 7: Protein engineering
- Lect. 8: Protein structure: historical perspective
- Lect 9: Methods for the determination of protein 3D structures
- Lect 10: Protein folding and unfolding: misfolding disorders
Practical course :
· Protein engineering
· Expression, purification and characterisation of recombinant enzymes
· Structural analysis of biomedically-relevant enzymes; mechanism of action of
compounds targeting blood clotting, HIV infection and hypercholesterolemia.
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