Molecular Biotechnology

October 12-23

From protein to gene, from gene to protein

 

 

 

Paula Veríssimo

Sandra Ribeiro

Sukalyan Chaterjee

Rui Cruz

Raquel Vinhas

 

Lab. Biotecnologia Molecular do CNC

Dep. Bioquímica, Universidade de Coimbra

IBMC

 

Lectures:

• Lect.1 Cloning strategies
• Lect.2 Expression of cloned genes in prokaryotes
• Lect. 3, 4: Expression of cloned genes in eukaryotes  
• Lect. 5: Recombinant protein production
• Lect. 6: Protein purification
• Lect. 7: Protein engineering
• Lect. 8: Protein structure: historical perspective
• Lect 9: Methods for the determination of protein 3D structures
• Lect 10: Protein folding and unfolding: misfolding disorders

 

 

 

Practical course :

 

·         Protein engineering

·         Expression, purification and characterisation of recombinant enzymes

·         Structural analysis of biomedically-relevant enzymes; mechanism of action of

compounds targeting blood clotting, HIV infection and hypercholesterolemia.

 

 

 

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